Comparison of α-Smooth Muscle Actin (α-SMA) and Collagen Type III Expression after Conjunctival Excision Between Platelet-Rich Fibrin (PRF) Membrane and Conjunctival Autograft Treatment: An Animal Model Experimental Study on Oryctolagus cuniculus

Doi: 10.36351/pjo.v40i2.1760

Authors

  • Indri Wahyuni
  • Reni Prastyani
  • Agung Bhakti Wiratama
  • Ismi Zuhria
  • Thomas Valentinus Widiyatno

DOI:

https://doi.org/10.36351/pjo.v40i2.1760

Abstract

Purpose: To evaluate the impact of Platelet-Rich Fibrin (PRF) membrane and conjunctival autografts on α-Smooth Muscle Actin (α-SMA) and type III collagen expression following conjunctival excision in Oryctolagus cuniculus.

Study Design: Experimental study with a randomized post-test only design.

Place and Duration of Study: Airlangga University, Surabaya in November 2022.

Methods: Twenty rabbit eyes were categorized into two groups: those sutured with PRF membrane and those sutured with conjunctival autografts after temporal conjunctival excision. The study assessed α-SMA and type III collagen expression through the Immunoreactive Score (IRS) method.Data analysis is conducted using appropriate statistical methods, such as the t-test or Mann-Whitney U test, to compare the expression of α-SMA and collagen type III between the two groups.

Results: The data for each sample represented the average IRS value observed at 200x magnification. In the conjunctival autograft group, the mean IRS of α-SMA expression was 5.52 ± 0.84,

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Published

01-04-2024

How to Cite

1.
Indri Wahyuni, Reni Prastyani, Agung Bhakti Wiratama, Ismi Zuhria, Thomas Valentinus Widiyatno. Comparison of α-Smooth Muscle Actin (α-SMA) and Collagen Type III Expression after Conjunctival Excision Between Platelet-Rich Fibrin (PRF) Membrane and Conjunctival Autograft Treatment: An Animal Model Experimental Study on Oryctolagus cuniculus: Doi: 10.36351/pjo.v40i2.1760. pak J Ophthalmol [Internet]. 2024 Apr. 1 [cited 2024 Jul. 14];40(2). Available from: https://pjo.org.pk/index.php/pjo/article/view/1760

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